Detection: all the 4 main subspecies of Xylella fastidiosa: X. fastidiosa subsp. fastidiosa, X. fastidiosa multiplex, X. fastidiosa subsp. sandyi and X. fastidiosa subsp. pauca, without any distinction.
Matrix: Plant petioles and central veins, non-woody branches or stems, vascular tissues of woody-branches (xylem).
Other information: set based on Harper and al., 2010, Erratum 2013. A verification was performed by Qualiplante (data not published) and the performance characteristics of the set are the same as the original publication.
The X. fastidiosa target sequence is located in the gene coding for the 16S rRNA-processing RimM protein. The Internal Control (IC) has been designed on the COX gene.
No cross reactions were observed from other bacterial species as Xanthomonas axonopodis pv. aurantifolii, X. campestris pv. citri, X. arboricola pv. fragariae, Pseudomonas syringae pv. persicae, Pantoea agglomerans, Agrobacterium tumefaciens and Spiroplasma citri.
The real-time test of Harper et al., 2010, Erratum 2013 is recommended by the European and Mediterranean Plant Protection Organization (www.eppo.int) – PM7/24, Bulletin (2019) 49 (2), 175–227, by the CRSFA of Bari (Current tools for the detection of Xylella fastidiosa in host plants and vectors, 23-24 October 2014) and by ANSES / LSV / MA 039 version 5, Juillet 2020.
Taq-Man® technology, FAM and Cy5 fluorophore.
Included reagents: Direct Master mix (D-MM), Negative Control (NC), Positive Control (PC).