Nested PCR Xfast - 24 tests / Nested PCR Xfast - 96 tests

Detection: all the 4 main subspecies of Xylella fastidiosa.

Matrix: Plant petioles and central veins, non-woody branches or stems, vascular tissues of woody-branches (xylem), and insects.

Other information: kit based on the amplification of a part of two conserved genes (16S rRNA gene and partial sequence of gyrB gene).

The PCR kit was tested on different strains of X. fastidiosa isolated from different plant hosts as grapevine, citrus, coffee, plum, hibiscus, almond, elm, ragweed, and non-targeted organisms, confirming 100% of specificity.

No cross-reactions were observed for: Xanthomonas albilineans, Xanthomonas axonopodis pv. citri, Xanthomonas bromi, Xanthomonas campestris pv. campestris, Xanthomonas campestris pv. vesicatoria, Xanthomonas campestris pv. cassavae, Xanthomonas campestris pv. manihotis, Xanthomonas campestris pv. citri, Xanthomonas campestris pv. malvacearum, Xanthomonas campestris pv. citrumelo, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. phaseoli, Xanthomonas cordiae, Xanthomonas curcubitae, Xanthomonas hyacinthi, Xanthomonas melonis, Xanthomonas translucens pv. translucens, Xanthomonas vasicola pv. holcicola, Curtobacterium flaccunfaciens, Enterobacter cloacae, Methylbacterium extorquens, Methylbacterium mesophilicum, Methylbacterium radiotolerans, Methylbacterium zatmanii, Pantoea agglomerans, Pseudomonas aeruginosa, Pseudomonas aureofaciens, Pseudomonas choloraphis, Pseudomonas fluorescens, Pseudomonas mendocina, Pseudomonas putida, Pseudomonas syringae pv. syringae, Agrobacterium tumefaciens, Candidatus liberibater africanus, Erwinia carotovora, Escherichia coli.

Included reagents: Direct Master Mix, Nested Master Mix, Negative control, Positive control.


Targeted pathogen:

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