Detection: Xanthomonas axonopodis pv. dieffenbachiae on anthurium.
Matrices: Bacterial culture or plant extracts, symptomatic and asymptomatic leaf.
Other information: kit developed and optimized by Qualiplante according to Robène-Soustrade et al. (2006). Under CIRAD license.
The PCR primers used in this Nested End-Point PCR method were selected from the SCAR sequence of Xad strain LMG695 and complemented some bases of the SCAR sequence.
This method is referred in the Appendix 4 of the PM7/23 (2) Xanthomonas axonopodis pv. dieffenbachiae, European and Mediterranean Plant Protection Organization Bulletin (2009) 39, 393-402.
The detection threshold obtained with pure cultures or plant extracts (103 CFU ml-1) allowed detection of the pathogen from symptomless contaminated plant. Internal validation data of the method are available on the ANSES/LSV/MA 028 – Version 3, September 2019 realized in 2008 by ANSES. The performance characteristics obtained are:
Kit referred in ANSES/LSV/MA 028.
Included reagents: Direct Master Mix, Nested Master Mix, Negative Control, Positive Control.