Universal Phytoplasma
Nested PCR Uniphy - 24 tests / Nested PCR Uniphy - 96 tests
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References :
- 24 tests: nPCR Uniphy 24
- 96 tests: nPCR Uniphy 96 - Crop: Fruit Trees and Small Fruits, Grapevines, Ornamentals, Vegetables
- Pathogen: Phytoplasma
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Method:
Nested End-Point PCR
Detection: Universal Phytoplasma (amplification of the 16S rRNA gene of any known phytoplasma).
Matrix: Leaves with symptoms knowing that phytoplasmas reside almost exclusively in the phloem sieve elements (cambium, petioles, midveins). Although phytoplasmas can be detected in roots and bark scrapings of dormant trees, generally it is best to test for phytoplasmas at the end of summer.
Other information: The most commonly used primers are the P1/P7 (Deng and Hiruki, 1991; Schneider et al., 1995) and R16F2n/R16R2 (Lee et al., 1993; Gundersen and Lee, 1996) primer pairs, which are used in this kit. The primer pair used for the 1st amplification amplifies a PCR product that contains the entire 16S rRNA gene as well as the 16S/23S rRNA spacer region. This primer pair was used by Smart et al. (1996) to amplify the 16S/23S rRNA spacer region of 10 different plytoplasma groups. Gundersen and Lee (1996) evaluated the R16F2n/R16R2 primers with representative phytoplasmas for 10 distinct 16 rRNA groups. Since then, both primer pairs have been used to characterize numerous new phytoplasma groups.
Included reagents: Direct Master Mix, Nested Master Mix, Negative control, Positive control.
Targeted pathogen:
Universal Phytoplasma
