Detection: Prunus necrotic ringspot virus on Prunus.
Matrices: Leaves, flowers, dormant buds or bark of young shoots.
Other information: kit developed and optimized by Qualiplante according to Candresse et al. (1998). A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication.
The primer pair was designed on highly conserved regions of the coat protein gene.
A cross-reaction with Apple mosaic virus (ApMV), another Ilarvirus, has been showed.
Included reagents: Direct Master Mix, RT Enzyme, Negative Control, Positive Control.