Plum pox virus
PCR PPV - 24 tests / PCR PPV - 96 tests
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References :
- 24 tests: PCR PPV 24
- 96 tests: PCR PPV 96 - Crop: Fruit Trees and Small Fruits
- Pathogen: Virus
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Method:
One-Step RT-PCR - Qualiplante method
Detection: Plum pox virus responsible of Sharka disease on Prunus.
Matrices: Plant leaves, flowers and buds.
Other information: kit based on Wetzel et al. (1991b). A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication. Moreover, the performance of the method was evaluated during an Italian project and obtains the following validation data on symptomatic plants collected in spring:
– sensibility: 100%
– specificity: 100%
– relative accuracy: 100%
– analytical sensitivity: 10-6
– analytical specificity: 100%
– repeatability: 100%
– reproducibility: 92,03%.
The sensitivity and the relative accuracy decrease when plants collected in spring are not symptomatic and (66%;83%) and when plants are collected in
winter (65%, 82%).
The primer pair was designed on homologous regions of different PPV strains (PPV-D, PPV-M and PPV ElAmar) and amplifies a 243 bp product.
This method is referred in the Appendix 3 of the EPPO PM7/032 (1).
Included reagents: Direct Master Mix, RT Enzyme, Negative Control, Positive Control.
Targeted pathogen:
Plum pox virus
