Detection: Pepino mosaic virus on tomato.
Matrix: Leaves of tomato plants or tomato seeds. For reliability, subsamples of no more than 250 seeds should be used in an individual test.
Other information: set developed and optimized by Qualiplante according to Ling et al., 2007. A verification was performed by Qualiplante (data not published) and the performance characteristics of the set are the same as the original publication.
The test was designed against the 5 strains of PepMV covered by 11 isolates: EU-tom, Ch-1, Ch-2, Peruvian and US-2 strains. The method was evaluated in a ring performance study on tomato seeds in 2009 as part of the EU-PRA PEPEIRA project.
The test could detect one naturally infested seed in 5.000.
The One-Step Real-Time RT-PCR test of Ling et al., 2007 is referred in the Appendix 4 of the PM7/113 (1) Pepino mosaic virus European and Mediterranean Plant Protection Organization Bulletin (2013) 43 (1), 94-104 and recommended by Progetto Aron-Arnadia – Armonizzazione Protocollo diagnostico per Pepino mosaic virus (PepMV) su pomodoro, Progetto Strateco, Anno 2012.
Taq-Man® technology, FAM fluorophore.
Included reagents: Direct Master Mix (DM), RT-Enzyme (RT), Negative Control (NC), Positive Control (PC).