PCR PepMV - 24 tests / PCR PepMV - 96 tests

Detection: Pepino mosaic viruson tomato.

Matrix: Seeds, leaves and fruit pulp of tomato plants.

Other information: kit developed and oprimized by Qualiplante according to Ling et al. (2008). A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication.

The primer pair was designed on the TGB2-3 genomic region.

The kit enables the detection of the 4 strain groups: European (EU), US1, US2 and Ch2.

Validation data of the method are available from a study realized in 2012 during Progetto Aron-Arnadia – Armonizzazione Protocollo diagnostico per Pepino mosaic virus (PepMV) su pomodoro, Progetto Strateco. The performance characteristics obtained are:

  • Diagnostic sensitivity: 91%.
  • Diagnostic specificity: 100%.
  • Accuracy: 93%.
  • Analytical sensitivity: 10-5 for seeds, 10-8 for fruit pulp, 10-6 for leaves.
  • Analytical specificity: 100%.
  • Repeatability: 100%.
  • Reproducibility: 98%.

Included reagents: Direct Master Mix, RT-Enzyme, Negative control, Positive control.


Targeted pathogen:

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