Detection: Pepino mosaic viruson tomato.
Matrix: Seeds, leaves and fruit pulp of tomato plants.
Other information: kit developed and oprimized by Qualiplante according to Ling et al. (2008). A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication.
The primer pair was designed on the TGB2-3 genomic region.
The kit enables the detection of the 4 strain groups: European (EU), US1, US2 and Ch2.
Validation data of the method are available from a study realized in 2012 during Progetto Aron-Arnadia – Armonizzazione Protocollo diagnostico per Pepino mosaic virus (PepMV) su pomodoro, Progetto Strateco. The performance characteristics obtained are:
Included reagents: Direct Master Mix, RT-Enzyme, Negative control, Positive control.