Detection: Grapevine virus A on grapevine.
Matrix: Grapevine leaves collected outside hot periods (for example in September/October) and bark scrapings from dormant canes.
Other information: kit developed and optimized by Qualiplante according to Margaria et al. 2009 and Osman and Rowhani, 2008. A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication.
The CP (Coat Protein) gene was used to design primers and probes.
Taq-Man® technology, FAM fluorophore.
Included reagents: Direct Master Mix,RT-Enzyme, Negative Control, Positive Control.