Detection: Grapevine fleck virus on grapevine.
Matrices: Grapevine leaves collected outside hot periods (for example in September/October) and bark scrapings from dormant canes.
Other information: kit developed and optimized by Qualiplante according to Osman et al., 2007. A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication.
The performance of the method has been demonstrated using several nucleic acids extraction procedures and a wide range of geographically distributed isolates (South Africa, Europe, Australia, Asia, Latin America and United States). The RdRp (RNA-dependent RNA polymerase) gene was used to design primers and probes.
Taq-Man® technology, FAM fluorophore.
Included reagents: Direct Master Mix, RT-Enzyme, Negative Control, Positive Control.