Detection: Grapevine fanleaf virus on grapevine.
Matrices:Grapevine leaves collected outside hot periods (for example in May/June) and bark scrapings from dormant canes.
Other information: kit developed and optimized by Qualiplante according to Osman and Rowhani, 2006. A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication.
The RNA2-polyprotein CP gene was used to design primers and probes.
Taq-Man® technology, FAM fluorophore.
Included reagents: Direct Master Mix, RT-Enzyme, Negative Control, Positive Control.