Detection: Potyvirus genus.
Matrix: Harvest fresh plant leaves tissues and tuber samples.
Other information: kit developed and optimized by Qualiplante according to Zheng et al., 2010. A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication. Primers were designed to amplify 2 conserved sites in the NIb (nuclear inclusion protein b) region.
RNA from 40 potyvirus isolates were tested. The NIb primers detected all the following potyviruses: Apium virus Y, Bean common mosaic virus, Bean yellow mosaic virus, Carrot virus Y, Ceratobium mosaic virus, Clover yellow vein virus, Cowpea aphid-borne mosaic virus, Dasheen mosaic virus, Freesia mosaic virus, Johnsongrass mosaic virus, Papaya ringspot virus, Passion fruit woodiness virus, Peanut mottle virus, Potato virus Y, Sugarcane mosaic virus, Sweet potato feathery mottle virus, Turnip mosaic virus, Watermelon mosaic virus, Zucchini yellow mosaic virus, Wheat streak mosaic virus, Banana bract mosaic virus, Onion yellow dwarf virus, Sarcochilus virus Y and Wisteria vein mosaic virus.
Included reagents: Direct Master Mix, Negative Control, Positive Control.