Detection: Candidatus phytoplasma vitis, responsible of Flavescence dorée and Candidatus phytoplasma solani, responsible of Bois noir on grapevine, with distinction of the two phytoplasma.
Matrices: Grapevine leaves, preferably primary veins and petioles and bark scrapings from dormant canes. It is preferable to test plant tissues with vine yellows symptoms.
Other information: kit developed according to the last French Official Method MOA006A-1b : détection des phytoplasmes de la vigne du groupe 16SrV (flavescence dorée) et du groupe 16SrXII (bois noir) PCR multiplex gigogne – ANSES.
This Duplex Nested End-Point PCR method includes for the 1st amplification specific primer sets for the detection of each phytoplasma groups (FD9f/FD9r and STOL11f2/STOL11r1) and for the 2nd amplification specific primer sets for each of the phytoplasma groups searched with specific sequences for the 1st PCR product (FD9r2/FD9f3b and STOL11f3/STOL11r2).
This method was evaluated during the EUPHRESCO Grafdepi I project (results available from www.euphresco.net/media/project_
Included reagents: Direct Master Mix, Nested Master Mix, Negative Control, Positive Control.