Candidatus phytoplasma solani
Candidatus phytoplasma vitis

Nested PCR FD BN - 24 tests / Nested PCR FD BN - 96 tests

References :
- 24 tests: nPCR FDBN 24
- 96 tests: nPCR FDBN96
Crop: Grapevines
Pathogen: Phytoplasma
Method:
Duplex Nested End-Point

Detection: Candidatus phytoplasma vitis, responsible of Flavescence dorée and Candidatus phytoplasma solani, responsible of Bois noir on grapevine, with distinction of the two phytoplasma.

Matrices: Grapevine leaves, preferably primary veins and petioles and bark scrapings from dormant canes. It is preferable to test plant tissues with vine yellows symptoms.

Other information: kit developed according to the last French Official Method MOA006A-1b : détection des phytoplasmes de la vigne du groupe 16SrV (flavescence dorée) et du groupe 16SrXII (bois noir) PCR multiplex gigogne – ANSES.

This Duplex Nested End-Point PCR method includes for the 1^st amplification specific primer sets for the detection of each phytoplasma groups (FD9f/FD9r and STOL11f2/STOL11r1) and for the 2^nd amplification specific primer sets for each of the phytoplasma groups searched with specific sequences for the 1^st PCR product (FD9r2/FD9f3b and STOL11f3/STOL11r2).

This method was evaluated during the EUPHRESCO Grafdepi I project (results available from www.euphresco.net/media/project_
reports/grafdepi_final_report.pdf).

Included reagents: Direct Master Mix, Nested Master Mix, Negative Control, Positive Control.