Detection: Candidatus phytoplasma vitis, responsible of Flavescence dorée on grapevine.
Matrix: Grapevine leaves, preferably primary veins and petioles. Other parts of grapevine tissues can be analyzed, for example vine stocks, or insects’ vectors, using specific nucleic acids extraction’s methods.
Other information: kit based on an analysis method developed by the company International Plant Analysis and Diagnostics (www.ipadlab.eu).
The primers and probes were designed on the rpl14 FD gene. The primers and probes sequences and their use in diagnostic tests are the subject of a PCT patent application by IpadLab (PCT/IB2010/053563). This Duplex Real-Time PCR kit offers a specific and sensitive method to detect 16SrV phytoplasmas including FD, as well as an endogenous control designed on the COX gene.
Taq-Man® technology, FAM (FD) and Cy5 (Internal Control) fluorophores.
Validation data of the method are available from a test performance study realized in 2013 (Euphresco Grafdepi project). This method is also referred in the Appendix 6 of the PM7/079 (2) Grapevine Flavescence dorée phytoplasma, European and Mediterranean Plant Protection Organization Bulletin (2016) 46 (1), 78-93.
Kit referred in PM7/079 (2).
Included: Direct Master Mix, Negative Control, Positive Control.