PCR.CMV set 24 / PCR CMV set 96

Detection: Clavibacter michiganensis subsp. michiganensis.

Matrix: Seeds and bacterial isolates from infected plants and seeds.

Other information: set developed and optimized by Qualiplante according to Pastrik & Rainey, 1999. A verification was performed by Qualiplante (data not published) and the performance characteristics of the set are the same as the original publication. Based on the amplification of the PSA-8/PSA-R primers.

It was possible to detect the pathogen in DNA extracts from 50 seeds containing 1×103 bacteria.

The PCR test of Pastrik & Rainey, 1999 is recommended by the European and Mediterranean Plant Protection Organization (www.eppo.int). in the Appendix 5 of the PM7/42 (3) (2016) Bulletin 46, 202– 225.

Included reagents: Direct Master Mix (DM), Negative Control (NC), Positive Control (PC).

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