Detection: Clavibacter michiganensis subsp. michiganensis.
Matrix: Seeds and bacterial isolates from infected plants and seeds.
Other information: kit developed and optimized by Qualiplante according to Pastrik & Rainey, 1999. A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication. Based on the amplification of the PSA-8/PSA-R primers.
It was possible to detect the pathogen in DNA extracts from 50 seeds containing 1×103 bacteria.
The PCR test of Pastrik & Rainey, 1999 is recommended by the European and Mediterranean Plant Protection Organization (www.eppo.int) in the Appendix 5 of the PM7/42 (3) (2016) Bulletin 46, 202– 225.
Included reagents: Direct Master Mix, Negative Control, Positive Control.