Clavibacter michiganensis pv. michiganensis
PCR Cmm - 24 tests / PCR Cmm - 96 tests
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References :
- 24 tests: PCR Cmm 24
- 96 tests: PCR Cmm 96 - Crop: Vegetables
- Pathogen: Bacteria
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Method:
End-Point PCR
Detection: Clavibacter michiganensis subsp. michiganensis.
Matrix: Seeds and bacterial isolates from infected plants and seeds.
Other information: kit developed and optimized by Qualiplante according to Pastrik & Rainey, 1999. A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication. Based on the amplification of the PSA-8/PSA-R primers.
It was possible to detect the pathogen in DNA extracts from 50 seeds containing 1×103 bacteria.
The PCR test of Pastrik & Rainey, 1999 is recommended by the European and Mediterranean Plant Protection Organization (www.eppo.int) in the Appendix 5 of the PM7/42 (3) (2016) Bulletin 46, 202– 225.
Included reagents: Direct Master Mix, Negative Control, Positive Control.
Targeted pathogen:
Clavibacter michiganensis pv. michiganensis
