Detection: Cherry leaf roll virus on cherry tree.
Matrices: The distribution of the virus in a tree can be irregular; however, the detection from flowers, young leaves, old leaves, fruit, dormant wood, and roots is possible. The time of year when the sample is taken is also critical and can greatly affect the test results. Optimal sampling is during spring, or early summer, but can vary from season to season depending on the weather conditions. CLRV has been reported to be easily detectable throughout the year in tissue of male inflorescences, leaf buds, leaves, single seeds and cortical tissues of young twigs. The virus can also be detected in roots, meristems and within tubules in pollen, ovules and mature seeds.
Other information: kit developed and optimized by Qualiplante according to Werner et al. (1997). A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication.
The primer pair amplifies a 416 bp product of the CLRV coat protein gene.
Included reagents: Direct Master Mix, RT-Enzyme, Negative Control, Positive Control.