qPCR BN - 24 tests / qPCR BN - 96 tests

Detection: Candidatus phytoplasma solani, responsible of Bois noir on grapevine.

Matrix: Grapevine leaves, preferably primary veins and petioles. Other parts of grapevine tissues can be analyzed, for example vine stocks, or insects’ vectors, using specific nucleic acids extraction’s methods.

Other information: kit based on an analysis method developed by the company International Plant Analysis and Diagnostics (www.ipadlab.eu).

The primers and probes were designed on the rpl22-rps3 BN gene. The primers and probes sequences and their use in diagnostic tests are the subject of a PCT patent application by IpadLab (PCT/IB2010/053563). This Duplex Real-Time PCR kit offers a specific and sensitive method to detect 16SrXII phytoplasmas including BN, as well as an endogenous control designed on the COX gene.

Taq-Man® technology, VIC (BN) and Cy5 (Internal Control) fluorophores.

Validation data of the method are available from a test performance study realized in 2013 (Euphresco Grafdepi project). This method is also referred in the Appendix 6 of the PM7/079 (2) Grapevine Flavescence dorée phytoplasma, European and Mediterranean Plant Protection Organization Bulletin (2016) 46 (1), 78-93.

Kit referred in PM7/079 (2).

Included reagents: Direct Master Mix, Negative Control, Positive Control.


Targeted pathogen:

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