PCR AgVit set 24 / PCR AgVit set 96
Detection: Agrobacterium vitis and Agrobacterium tumefaciens, with discrimination.
Matrix: Grapevine tumors.
Other information: The method uses a combination of 3 specific primers sets that permit to amplify:
- the chromosomal polygalacturonase gene pehA used to identify vitis strains and to distinguish them from A. tumefaciens (466 bp DNA fragment),
- a region of virF gene coding for vitis nopaline and octopine pTi (382 bp),
- a region of VirD gene coding for vitis vitopine.
Included reagents: Direct Master Mix (DM), Negative Control (NC), Positive control (PC).
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