PCR AgVit set 24 / PCR AgVit set 96

Detection: Agrobacterium vitis and Agrobacterium tumefaciens, with discrimination.

Matrix: Grapevine tumors.

Other information: The method uses a combination of 3 specific primers sets that permit to amplify:

  • the chromosomal polygalacturonase gene pehA used to identify vitis strains and to distinguish them from A. tumefaciens (466 bp DNA fragment),
  • a region of virF gene coding for vitis nopaline and octopine pTi (382 bp),
  • a region of VirD gene coding for vitis vitopine.

Included reagents: Direct Master Mix (DM), Negative Control (NC), Positive control (PC).

USER GUIDE

 

Targeted pathogen:

Request a quote