This kit permits to detect Agrobacterium vitis and Agrobacterium tumefaciens and to distinguish the virulent and not virulent strain of the bacteria
Matrices: Grapevine tumors tissues, roots or wood canes
Other information: Permits to detect Agrobacterium vitis and Agrobacterium tumefaciens and to distinguish the virulent and not virulent strain of the bacteria. This Triplex End-Point PCR method uses a combination of 3 specific primers sets that permit to amplify:
- the chromosomal polygalacturonase gene pehA used to identify vitis strains and to distinguish them from A. tumefaciens,
- a region of virF gene coding for vitis nopaline and octopine pTi,
- a region of virD gene coding for vitis vitopine.
Included:
- Ready-to-use A.vitis PCR Master mix (2×48 tests),
- Positive Amplification Control (8 tests),
- Negative Amplification Control (8 tests),
- User Guide,
- Certificate of Quality
Targeted pathogen:
Agrobacterium tumefaciens
Agrobacterium vitis
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