This kit permits to detect Agrobacterium vitis and Agrobacterium tumefaciens and to distinguish the virulent and not virulent strain of the bacteria

Matrices: Grapevine tumors tissues, roots or wood canes

Other information: Permits to detect Agrobacterium vitis and Agrobacterium tumefaciens and to distinguish the virulent and not virulent strain of the bacteria. This Triplex End-Point PCR method uses a combination of 3 specific primers sets that permit to amplify:

  • the chromosomal polygalacturonase gene pehA used to identify vitis strains and to distinguish them from A. tumefaciens,
  • a region of virF gene coding for vitis nopaline and octopine pTi,
  • a region of virD gene coding for vitis vitopine.

Included:

  • Ready-to-use A.vitis PCR Master mix (2×48 tests),
  • Positive Amplification Control (8 tests),
  • Negative Amplification Control (8 tests),
  • User Guide,
  • Certificate of Quality
Targeted pathogen:

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