Arabis mosaic virus
Grapevine fanleaf virus
Grapevine fleck virus
Grapevine leafroll associated virus-1
Grapevine leafroll associated virus-2
Grapevine leafroll associated virus-3
Grapevine virus A

PCR ArMV, GFLV, GFkV, GLRaV-1,2,3, GVA woRT - 24 tests / PCR ArMV, GFLV, GFkV, GLRaV-1,2,3, GVA woRT - 96 tests

References :
- 24 tests: PCR 7GV woRT 24
- 96 tests: PCR 7GVwoRT 96
Crop: Grapevines
Pathogen: Virus
Method:
Multiplex End-Point PCR - without RT-Enzyme

Detection: Arabis mosaic virus (ArMV), Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated virus -1 (GLRaV-1), -2 (GLRaV-2) and -3 (GLRaV-3), Grapevine virus A (GVA) and Grapevine fleck virus (GFkV) on grapevine.

Matrix:Grapevine leaves and bark scrapings from dormant canes.

Other information: kit developed and optimized by Qualiplante according to Gambino and Gribaudo, 2006. A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication.

This method was compared to ELISA method (3 different reagents) during Arnadia Italian project on 122 infected grapevine samples tested by 13 laboratories. The results of the ring-test were presented during the 17^th Congress of ICVG, Davis, California, in 2012.

Internal control. Without RT Master Mix and RT-Enzyme.

Included reagents: Direct Master Mix, Positive Control A, Positive Control B, Negative Control.